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N2 -Substituted 2'-Deoxyguanosine Triphosphate Derivatives as Selective Substrates for Human DNA Polymerase ?.


ABSTRACT: N2 -Alkyl-2'-deoxyguanosine triphosphate (N2 -alkyl-dGTP) derivatives with methyl, butyl, benzyl, or 4-ethynylbenzyl substituents were prepared and tested as substrates for human DNA polymerases. N2 -Benzyl-dGTP was equal to dGTP as a substrate for DNA polymerase?? (pol??), but was a poor substrate for pols??, ?, ?, ?, or ?. In?vivo reactivity was evaluated through incubation of N2 -4-ethynylbenzyl-dG with wild-type and pol?? deficient mouse embryonic fibroblasts. CuAAC reaction with 5(6)-FAM-azide demonstrated that only cells containing pol?? were able to incorporate N2 -4-ethynylbenzyl-dG into the nucleus. This is the first instance of a Y-family-polymerase-specific dNTP, and this method could be used to probe the activity of pol?? in?vivo.

SUBMITTER: Gowda AS 

PROVIDER: S-EPMC5394382 | BioStudies | 2017-01-01

REPOSITORIES: biostudies

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