Dataset Information


Sphingosine kinase 1-interacting protein is a novel regulator of glucose-stimulated insulin secretion.

ABSTRACT: Glucose-stimulated insulin secretion (GSIS) is essential in keeping blood glucose levels within normal range. GSIS is impaired in type 2 diabetes, and its recovery is crucial in treatment of the disease. We find here that sphingosine kinase 1-interacting protein (SKIP, also called Sphkap) is highly expressed in pancreatic ?-cells but not in ?-cells. Intraperitoneal glucose tolerance test showed that plasma glucose levels were decreased and insulin levels were increased in SKIP-/- mice compared to SKIP+/+ mice, but exendin-4-enhanced insulin secretion was masked. GSIS was amplified more in SKIP-/- but exendin-4-enhanced insulin secretion was masked compared to that in SKIP+/+ islets. The ATP and cAMP content were similarly increased in SKIP+/+ and SKIP-/- islets; depolarization-evoked, PKA and cAMP-mediated insulin secretion were not affected. Inhibition of PDE activity equally augmented GSIS in SKIP+/+ and SKIP-/- islets. These results indicate that SKIP modulates GSIS by a pathway distinct from that of cAMP-, PDE- and sphingosine kinase-dependent pathways.

PROVIDER: S-EPMC5429731 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC4921792 | BioStudies
| S-EPMC6635387 | BioStudies
| S-EPMC6152341 | BioStudies
| S-EPMC7185381 | BioStudies
| S-EPMC4562512 | BioStudies
| S-EPMC8635970 | BioStudies
| S-EPMC7260680 | BioStudies
| S-EPMC8471175 | BioStudies
| S-EPMC4191183 | BioStudies
| S-EPMC4058888 | BioStudies