Dataset Information


Mcl-1 regulates reactive oxygen species via NOX4 during chemotherapy-induced senescence.

ABSTRACT: Mcl-1, a Bcl-2 family member, is highly expressed in a variety of human cancers and is believed to enhance tumorigenic potential and chemotherapy resistance through the inhibition of apoptosis and senescence. We previously reported that Mcl-1's regulation of chemotherapy-induced senescence (CIS) is dependent on its ability to prevent reactive oxygen species (ROS) generation. In this report, we demonstrate that Mcl-1-regulated CIS requires not only ROS, but specifically mitochondrial ROS, and that these events are upstream of activation of the DNA damage response, another necessary step toward senescence. Mcl-1's anti-senescence activity also involves the unique ability to inhibit ROS formation by preventing the upregulation of pro-oxidants. Specifically, we found that NADPH oxidases (NOXs) are regulated by Mcl-1 and that NOX4 expression in particular is a required step for CIS induction that is blocked by Mcl-1. Lastly, we illustrate that by preventing expression of NOX4, Mcl-1 limits its availability in the mitochondria, thereby lowering the production of mitochondrial ROS during CIS. Our studies not only define the essential role of Mcl-1 in chemoresistance, but also for the first time link a key pro-survival Bcl-2 family member with the NOX protein family, both of which have significant ramifications in cancer progression.

SUBMITTER: Demelash A 

PROVIDER: S-EPMC5438639 | BioStudies | 2017-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC2762686 | BioStudies
2017-01-01 | S-EPMC5488022 | BioStudies
1000-01-01 | S-EPMC6203707 | BioStudies
2019-01-01 | S-EPMC6374999 | BioStudies
2015-01-01 | S-EPMC4423734 | BioStudies
2018-01-01 | S-EPMC5766150 | BioStudies
2019-01-01 | S-EPMC6831838 | BioStudies
2017-01-01 | S-EPMC5632907 | BioStudies
2012-01-01 | S-EPMC3392471 | BioStudies
1000-01-01 | S-EPMC5341811 | BioStudies