Dataset Information


Polycomb Repressive Complex 2-Mediated Chromatin Repression Guides Effector CD8+ T Cell Terminal Differentiation and Loss of Multipotency.

ABSTRACT: Understanding immunological memory formation depends on elucidating how multipotent memory precursor (MP) cells maintain developmental plasticity and longevity to provide long-term immunity while other effector cells develop into terminally differentiated effector (TE) cells with limited survival. Profiling active (H3K27ac) and repressed (H3K27me3) chromatin in naive, MP, and TE CD8+ T cells during viral infection revealed increased H3K27me3 deposition at numerous pro-memory and pro-survival genes in TE relative to MP cells, indicative of fate restriction, but permissive chromatin at both pro-memory and pro-effector genes in MP cells, indicative of multipotency. Polycomb repressive complex 2 deficiency impaired clonal expansion and TE cell differentiation, but minimally impacted CD8+ memory T cell maturation. Abundant H3K27me3 deposition at pro-memory genes occurred late during TE cell development, probably from diminished transcription factor FOXO1 expression. These results outline a temporal model for loss of memory cell potential through selective epigenetic silencing of pro-memory genes in effector T cells.


PROVIDER: S-EPMC5457165 | BioStudies | 2017-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-03-13 | GSE89037 | GEO
2015-09-15 | E-GEOD-72408 | ArrayExpress
2014-01-01 | S-EPMC4479393 | BioStudies
2016-01-01 | S-EPMC4947817 | BioStudies
2020-01-01 | S-EPMC7282668 | BioStudies
2020-01-01 | S-EPMC7338451 | BioStudies
2018-01-01 | S-EPMC6748641 | BioStudies
2015-01-01 | S-EPMC4237716 | BioStudies
2011-01-01 | S-EPMC3991478 | BioStudies
2021-01-01 | S-EPMC7818084 | BioStudies