Unknown

Dataset Information

0

Overexpression of miR-24 Is Involved in the Formation of Hypocoagulation State after Severe Trauma by Inhibiting the Synthesis of Coagulation Factor X.


ABSTRACT: Dysregulation of microRNAs may contribute to the progression of trauma-induced coagulopathy (TIC). We aimed to explore the biological function that miRNA-24-3p (miR-24) might have in coagulation factor deficiency after major trauma and TIC.15 healthy volunteers and 36 severe trauma patients (Injury Severity Score ≥ 16 were enrolled. TIC was determined as the initial international normalized ratio >1.5. The miR-24 expression and concentrations of factor X (FX) and factor XII in plasma were measured. In vitro study was conducted on L02 cell line.The plasma miR-24 expression was significantly elevated by 3.17-fold (P = 0.043) in major trauma patients and reduced after 3 days (P < 0.01). The expression level was significantly higher in TIC than in non-TIC patients (P = 0.040). Multivariate analysis showed that the higher miR-24 expression was associated with TIC. The plasma concentration of FX in TIC patients was significantly lower than in the non-TIC ones (P = 0.030) and controls (P < 0.01). A negative correlation was observed between miR-24 and FX. miR-24 transduction significantly reduced the FX level in the supernatant of L02 cells (P = 0.030).miR-24 was overexpressed in major trauma and TIC patients. The negative correlation of miR-24 with FX suggested the possibility that miR-24 might inhibit the synthesis of FX during TIC.

PROVIDER: S-EPMC5488151 | BioStudies | 2017-01-01

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC6850475 | BioStudies
2015-01-01 | S-EPMC4408845 | BioStudies
| S-EPMC6043648 | BioStudies
2015-01-01 | S-EPMC4176550 | BioStudies
2016-01-01 | S-EPMC5000842 | BioStudies
| S-EPMC6487715 | BioStudies
| S-EPMC7780743 | BioStudies
| S-EPMC4518517 | BioStudies
| S-EPMC6980474 | BioStudies
| S-EPMC6977230 | BioStudies