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How to speed up the polymerase chain reaction.


ABSTRACT: Reducing the time taken to run qPCR assays on today's qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.

PROVIDER: S-EPMC5496742 | BioStudies |

REPOSITORIES: biostudies

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