Dataset Information


Identification of the miRNA-mRNA regulatory network of small cell osteosarcoma based on RNA-seq.

ABSTRACT: Small cell osteosarcoma (SCO) is a rare subtype of osteosarcoma characterized by highly aggressive progression and a poor prognosis. The miRNA and mRNA expression profiles of peripheral blood mononuclear cells (PBMCs) were obtained in 3 patients with SCO and 10 healthy individuals using high-throughput RNA-sequencing. We identified 37 dysregulated miRNAs and 1636 dysregulated mRNAs in patients with SCO compared to the healthy controls. Specifically, the 37 dysregulated miRNAs consisted of 27 up-regulated miRNAs and 10 down-regulated miRNAs; the 1636 dysregulated mRNAs consisted of 555 up-regulated mRNAs and 1081 down-regulated mRNAs. The target-genes of miRNAs were predicted, and 1334 negative correlations between miRNAs and mRNAs were used to construct an miRNA-mRNA regulatory network. Dysregulated genes were significantly enriched in pathways related to cancer, mTOR signaling and cell cycle signaling. Specifically, hsa-miR-26b-5p, hsa-miR-221-3p and hsa-miR-125b-2-3p were significantly dysregulated miRNAs and exhibited a high degree of connectivity with target genes. Overall, the expression of dysregulated genes in tumor tissues and peripheral blood samples of patients with SCO measured by quantitative real-time polymerase chain reaction corroborated with our bioinformatics analyses based on the expression profiles of PBMCs from patients with SCO. Thus, hsa-miR-26b-5p, hsa-miR-221-3p and hsa-miR-125b-2-3p may be involved in SCO tumorigenesis.

PROVIDER: S-EPMC5522085 | BioStudies | 2017-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC4369814 | BioStudies
2019-01-01 | S-EPMC6676736 | BioStudies
| S-EPMC7540039 | BioStudies
2019-01-01 | S-EPMC6790409 | BioStudies
2018-01-01 | S-EPMC5858605 | BioStudies
2020-01-01 | S-EPMC7844321 | BioStudies
2020-01-01 | S-EPMC7142269 | BioStudies
2020-01-01 | S-EPMC7533449 | BioStudies
1000-01-01 | S-EPMC4119883 | BioStudies
2019-01-01 | S-EPMC6712721 | BioStudies