Dataset Information


Targeted migration and differentiation of engrafted neural precursor cells in amyloid beta-treated hippocampus in rats.

ABSTRACT: To observe the migration and differentiation of the neural precursor cells (NPCs) that derived from murine embryonic stem cells (ESCs) when they were transplanted into amyloid beta (A beta)-treated rat hippocampus.MESPU35, a murine ESC cell line that express the enhanced green fluorescent protein (EGFP), was induced differentiation into nestin-positive NPCs by modified serum-free methods. The A beta plaques and the differentiation of the grafted cells were observed by immunofluorescent staining.Comparing 16 weeks with 4 weeks post-transplantation, the migration distance increased about 5 times; the rate of migratory NPCs differentiating into glial fibrillary acidic protein (GFAP)-positive cells kept rising from (30.41+/-1.45) % to (49.25+/-1.23) %, and the rate of NPCs differentiating into neurofilament 200 (NF200) positive cells increased from (16.68+/-0.95) % to (27.94+/-1.21) %. Meanwhile, the GFAP-positive cells targeting to the ipsilateral side of A beta plaques increased from 60.2% to 81.3%, while the NF200-positive cells increased from 61.3% to 84.1%. The migration distance had significant positive linear correlations to the neuronal differentiation rate (r = 0.991) and to the astrocytic differentiation rate (r = 0.953).Engrafted NPCs migrate targetedly to the A beta injection site and differentiate into neurons and astrocytes.


PROVIDER: S-EPMC5550573 | BioStudies | 2007-01-01

SECONDARY ACCESSION(S): 10.1007/s12264-007-0039-5

REPOSITORIES: biostudies

Similar Datasets

2019-01-01 | S-EPMC6921476 | BioStudies
1000-01-01 | S-EPMC6266631 | BioStudies
1000-01-01 | S-EPMC2755676 | BioStudies
2008-01-01 | S-EPMC2527128 | BioStudies
2017-01-01 | S-EPMC5426660 | BioStudies
1000-01-01 | S-EPMC3973708 | BioStudies
1000-01-01 | S-EPMC5819232 | BioStudies
1000-01-01 | S-EPMC4828673 | BioStudies
2016-01-01 | S-EPMC4699090 | BioStudies
2009-01-01 | S-EPMC2749153 | BioStudies