Unknown

Dataset Information

0

Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide.


ABSTRACT: A goal for an HIV-1 vaccine is to overcome virus variability by inducing broadly neutralizing antibodies (bnAbs). One key target of bnAbs is the glycan-polypeptide at the base of the envelope (Env) third variable loop (V3). We have designed and synthesized a homogeneous minimal immunogen with high-mannose glycans reflective of a native Env V3-glycan bnAb epitope (Man9-V3). V3-glycan bnAbs bound to Man9-V3 glycopeptide and native-like gp140 trimers with similar affinities. Fluorophore-labeled Man9-V3 glycopeptides bound to bnAb memory B cells and were able to be used to isolate a V3-glycan bnAb from an HIV-1-infected individual. In rhesus macaques, immunization with Man9-V3 induced V3-glycan-targeted antibodies. Thus, the Man9-V3 glycopeptide closely mimics an HIV-1 V3-glycan bnAb epitope and can be used to isolate V3-glycan bnAbs.

SUBMITTER: Alam SM 

PROVIDER: S-EPMC5562351 | BioStudies | 2017-01-01

REPOSITORIES: biostudies

Similar Datasets

2018-01-01 | S-EPMC5856820 | BioStudies
2018-01-01 | S-EPMC5871869 | BioStudies
| S-EPMC5741509 | BioStudies
2020-01-01 | S-EPMC7294236 | BioStudies
2017-01-01 | S-EPMC5408352 | BioStudies
2017-01-01 | S-EPMC5562350 | BioStudies
2018-01-01 | S-EPMC5830440 | BioStudies
2020-01-01 | S-EPMC6960291 | BioStudies
2017-01-01 | S-EPMC5571269 | BioStudies
2019-01-01 | S-EPMC6597128 | BioStudies