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Dense sgRNA Library Construction Using a Molecular Chipper Approach.


ABSTRACT: Genetic screens using single-guide-RNA (sgRNA) libraries and CRISPR technology have been powerful to identify genetic regulators for both coding and noncoding regions of the genome. Interrogating functional elements in noncoding regions requires sgRNA libraries that are densely covering, and ideally inexpensive, easy to implement and flexible for customization. We present a Molecular Chipper protocol for generating dense sgRNA libraries from genomic regions of interest. This approach utilizes a combination of random fragmentation and a Type III restriction enzyme to derive a dense coverage of sgRNA library from input DNA.

SUBMITTER: Cheng J 

PROVIDER: S-EPMC5564686 | BioStudies | 2017-01-01

SECONDARY ACCESSION(S): 10.21769/BioProtoc.2373

REPOSITORIES: biostudies

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