Dataset Information


Necroptotic debris including damaged mitochondria elicits sepsis-like syndrome during late-phase tularemia.

ABSTRACT: Infection with Francisella tularensis ssp. tularensis (Ft) strain SchuS4 causes an often lethal disease known as tularemia in rodents, non-human primates, and humans. Ft subverts host cell death programs to facilitate their exponential replication within macrophages and other cell types during early respiratory infection (?72?h). The mechanism(s) by which cell death is triggered remains incompletely defined, as does the impact of Ft on mitochondria, the host cell's organellar 'canary in a coal mine'. Herein, we reveal that Ft infection of host cells, particularly macrophages and polymorphonuclear leukocytes, drives necroptosis via a receptor-interacting protein kinase 1/3-mediated mechanism. During necroptosis mitochondria and other organelles become damaged. Ft-induced mitochondrial damage is characterized by: (i) a decrease in membrane potential and consequent mitochondrial oncosis or swelling, (ii) increased generation of superoxide radicals, and (iii) release of intact or damaged mitochondria into the lung parenchyma. Host cell recognition of and response to released mitochondria and other damage-associated molecular patterns engenders a sepsis-like syndrome typified by production of TNF, IL-1?, IL-6, IL-12p70, and IFN-? during late-phase tularemia (?72?h), but are absent early during infection.


PROVIDER: S-EPMC5611684 | BioStudies | 2017-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2013-01-01 | S-EPMC3595284 | BioStudies
1000-01-01 | S-EPMC4358995 | BioStudies
2011-01-01 | S-EPMC3306246 | BioStudies
2010-01-01 | S-EPMC3111085 | BioStudies
1000-01-01 | S-EPMC6056872 | BioStudies
1000-01-01 | S-EPMC5235906 | BioStudies
2012-01-01 | S-EPMC3299770 | BioStudies
2016-01-01 | S-EPMC4807818 | BioStudies
2010-01-01 | S-EPMC2887844 | BioStudies
2013-01-01 | S-EPMC3754213 | BioStudies