Dataset Information


Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens.

ABSTRACT: Rapid and sensitive point-of-care diagnostics are of paramount importance for early detection of infectious diseases and timely initiation of treatment. Here, we present cellulose paper and flexible plastic chips with printed graphene-modified silver electrodes as universal point-of-care diagnostic tools for the rapid and sensitive detection of microbial pathogens or nucleic acids through utilizing electrical sensing modality and loop-mediated isothermal amplification (LAMP). We evaluated the ability of the developed paper-based assay to detect (i) viruses on cellulose-based paper microchips without implementing amplification in samples with viral loads between 106 and 108 copies per ml, and (ii) amplified HIV-1 nucleic acids in samples with viral loads between 10 fg ?l-1 and 108 fg ?l-1. The target HIV-1 nucleic acid was amplified using the RT-LAMP technique and detected through the electrical sensing of LAMP amplicons for a broad range of RNA concentrations between 10 fg ?l-1 and 108 fg ?l-1 after 40 min of amplification time. Our assay may be used for antiretroviral therapy monitoring where it meets the sensitivity requirement of the World Health Organization guidelines. Such a paper microchip assay without the amplification step may also be considered as a simple and inexpensive approach for acute HIV detection where maximum viral replication occurs.

SUBMITTER: Safavieh M 

PROVIDER: S-EPMC5695240 | BioStudies | 2017-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2016-01-01 | S-EPMC4855516 | BioStudies
2013-01-01 | S-EPMC3897712 | BioStudies
2015-01-01 | S-EPMC4687746 | BioStudies
2020-01-01 | S-EPMC7666441 | BioStudies
2015-01-01 | S-EPMC4439053 | BioStudies
2012-01-01 | S-EPMC3448666 | BioStudies
2012-01-01 | S-EPMC3285652 | BioStudies
2019-01-01 | S-EPMC6980338 | BioStudies
2013-01-01 | S-EPMC3578705 | BioStudies
2021-01-01 | S-EPMC7915047 | BioStudies