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Unraveling the Thermodynamics of the Folding and Interconversion of Human Telomere G-Quadruplexes.


ABSTRACT: Why human telomere DNA fragments fold into different G-quadruplex structures with parallel, hybrid, and antiparallel strand orientations depending on the temperature and concentration of co-solutes remains poorly understood. Similarly, the formation of intermediate structures along the folding or interconversion pathways is not well understood. Herein, we address these questions by introducing a conceptual framework, based on the global thermodynamic analysis of DSC and CD spectroscopy data, which led to a detailed description of the topological phase space (phase diagram) of the stability of the human telomere fragment 5'-AGGG(TTAGGG)3 -3' (Tel22). This framework clarifies the driving forces of quadruplex folding and interconversion processes over a wide range of temperatures and ion (K(+) , Na(+) ) and polyethylene glycol (PEG) concentrations and demonstrates their linkage to the human telomere DNA structural features.

SUBMITTER: Boncina M 

PROVIDER: S-EPMC5705027 | BioStudies | 2016-01-01

REPOSITORIES: biostudies

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