Unknown

Dataset Information

0

DRNA-seq transcriptional profiling of the FK506 biosynthetic gene cluster in Streptomyces tsukubaensis NRRL18488 and general analysis of the transcriptome.


ABSTRACT: FK506 (tacrolimus) is a valuable immunosuppressant produced by several Streptomyces strains. In the genome of the wild type producer Streptomyces tsukubaensis NRRL18488, FK506 biosynthesis is encoded by a gene cluster that spans 83.5 (kb). A whole transcriptome differential shotgun sequencing (dRNA-seq) of S. tsukubaensis was performed to analyze transcription at 2 different time points; before and during active FK506 production. In total, 8,914 transcription start sites were identified in either condition, which enabled precise determination of the 5'-UTR length of the corresponding transcripts as well as the identification of 2 consensus sequence motifs in the promoter regions. The transcription start sites of all gene operons within the FK506 cluster were identified, including 3 examples of leaderless RNA transcripts. These data provide detailed insight into the transcription of the FK506 biosynthetic gene cluster to support future regulatory studies, genetic manipulation, and industrial production.

SUBMITTER: Bauer JS 

PROVIDER: S-EPMC5785230 | BioStudies | 2017-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-07-05 | GSE92480 | GEO
2012-01-01 | S-EPMC3551636 | BioStudies
2019-10-16 | GSE97637 | GEO
2012-01-01 | S-EPMC3393489 | BioStudies
2016-01-01 | S-EPMC4823652 | BioStudies
2013-01-01 | S-EPMC3680238 | BioStudies
2020-02-08 | GSE110393 | GEO
2013-01-01 | S-EPMC3708917 | BioStudies
2010-01-01 | S-EPMC2863216 | BioStudies
2012-01-01 | S-EPMC3497360 | BioStudies