Unknown

Dataset Information

0

UCP1 deficiency increases adipose tissue monounsaturated fatty acid synthesis and trafficking to the liver.


ABSTRACT: Uncoupling protein-1 (UCP1) facilitates thermogenesis in brown and beige adipocytes and can promote energy expenditure by decreasing mitochondrial respiratory efficiency. Defects in UCP1 and brown adipose tissue thermogenesis subject animals to chronic cold stress and elicit compensatory responses to generate heat. How UCP1 regulates white adipose tissue (WAT) lipid biology and tissue crosstalk is not completely understood. Here, we probed the effect of UCP1 deficiency on FA metabolism in inguinal and epididymal WAT and investigated how these metabolic perturbations influence hepatic lipid homeostasis. We report that at standard housing temperature (21°C), loss of UCP1 induces inguinal WAT de novo lipogenesis through transcriptional activation of the lipogenic gene program and elevated GLUT4. Inguinal adipocyte hyperplasia and depot expansion accompany the increase in lipid synthesis. We also found that UCP1 deficiency elevates adipose stearoyl-CoA desaturase gene expression, and increased inguinal WAT lipolysis supports the transport of adipose-derived palmitoleate (16:1n7) to the liver and hepatic triglyceride accumulation. The observed WAT and liver phenotypes were resolved by housing animals at thermoneutral housing (30°C). These data illustrate depot-specific responses to impaired BAT thermogenesis and communication between WAT and liver in UCP1-/- mice.

SUBMITTER: Bond LM 

PROVIDER: S-EPMC5794418 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC4682086 | BioStudies
| S-EPMC6872148 | BioStudies
2020-01-01 | S-EPMC7059603 | BioStudies
2019-01-01 | S-EPMC6857924 | BioStudies
2018-01-01 | S-EPMC6235278 | BioStudies
2017-01-01 | S-EPMC5532220 | BioStudies
2019-01-01 | S-EPMC6521120 | BioStudies
2018-01-01 | S-EPMC5826329 | BioStudies
2016-01-01 | S-EPMC4972251 | BioStudies
1000-01-01 | S-EPMC4481421 | BioStudies