Unknown

Dataset Information

0

Galectin-1 is required for the regulatory function of B cells.


ABSTRACT: Galectin-1 (Gal-1) is required for the development of B cells in the bone marrow (BM), however very little is known about the contribution of Gal-1 to the development of B cell regulatory function. Here, we report an important role for Gal-1 in the induction of B cells regulatory function. Mice deficient of Gal-1 (Gal-1-/-) showed significant loss of Transitional-2 (T2) B cells, previously reported to include IL-10+ regulatory B cells. Gal-1-/- B cells stimulated in vitro via CD40 molecules have impaired IL-10 and Tim-1 expression, the latter reported to be required for IL-10 production in regulatory B cells, and increased TNF-? expression compared to wild type (WT) B cells. Unlike their WT counterparts, T2 and T1 Gal-1-/- B cells did not suppress TNF-? expression by CD4+ T cells activated in vitro with allogenic DCs (allo-DCs), nor were they suppressive in vivo, being unable to delay MHC-class I mismatched skin allograft rejection following adoptive transfer. Moreover, T cells stimulated with allo-DCs show an increase in their survival when co-cultured with Gal-1-/- T2 and MZ B cells compared to WT T2 and MZ B cells. Collectively, these data suggest that Gal-1 contributes to the induction of B cells regulatory function.

SUBMITTER: Alhabbab R 

PROVIDER: S-EPMC5807431 | BioStudies | 2018-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2019-01-01 | S-EPMC6735380 | BioStudies
2007-01-01 | S-EPMC2278022 | BioStudies
1000-01-01 | S-EPMC6213651 | BioStudies
2017-01-01 | S-EPMC5350155 | BioStudies
1000-01-01 | S-EPMC3973106 | BioStudies
2011-01-01 | S-EPMC3087832 | BioStudies
2018-01-01 | S-EPMC6190848 | BioStudies
2015-01-01 | S-EPMC4704704 | BioStudies
2015-01-01 | S-EPMC4324157 | BioStudies
1000-01-01 | S-EPMC4420608 | BioStudies