Unknown

Dataset Information

0

A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas.


ABSTRACT: Light-sensitivity is important for mollusc survival, as it plays a vital role in reproduction and predator avoidance. Light-sensitivity has been demonstrated in the adult Pacific oyster Crassostrea gigas, but the genes associated with light-sensitivity remain unclear. In the present study, we designed experiments to identify the genes associated with light-sensitivity in adult oysters. First, we assessed the Pacific oyster genome and identified 368 genes annotated with the terms associated with light-sensitivity. Second, the function of the four rhodopsin-like superfamily member genes was tested by using RNAi. The results showed that the highest level of mRNA expression of the vision-related genes was in the mantle; however, this finding is not true for all oyster genes. Interestingly, we also found four rhodopsin-like superfamily member genes expressed at an very high level in the mantle tissue. In the RNAi experiment, when one of rhodopsin-like superfamily member genes (CGI_1001253) was inhibited, the light-sensitivity capacity of the injected oysters was significantly reduced, suggesting that CGI_10012534 may be associated with light-sensitivity in the adult Pacific oyster.

SUBMITTER: Wu C 

PROVIDER: S-EPMC5868073 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

2019-01-01 | S-EPMC6555990 | BioStudies
2015-01-01 | S-EPMC4608825 | BioStudies
2017-03-22 | GSE94633 | GEO
2020-01-01 | S-EPMC7271890 | BioStudies
2016-01-01 | S-EPMC5040781 | BioStudies
2019-01-01 | S-EPMC6611504 | BioStudies
2018-01-01 | S-EPMC6278567 | BioStudies
2019-01-01 | S-EPMC6799575 | BioStudies
2017-01-01 | S-EPMC5594505 | BioStudies
2017-01-01 | S-EPMC5542438 | BioStudies