Dataset Information


Delayed and highly specific antibody response to nonstructural protein 1 (NS1) revealed during natural human ZIKV infection by NS1-based capture ELISA.

ABSTRACT: BACKGROUND:Zika virus (ZIKV) had spread rapidly in the past few years in southern hemisphere where dengue virus (DENV) had caused epidemic problems for over half a century. The high degree of cross-reactivity of Envelope (E) protein specific antibody responses between ZIKV and DENV made it challenging to perform differential diagnosis between the two infections using standard ELISA method for E protein. METHODS:Using an IgG capture ELISA, we investigated the kinetics of nonstructural protein 1 (NS1) antibody response during natural ZIKV infection and the cross-reactivity to NS1 proteins using convalescent sera obtained from patients infected by either DENV or ZIKV. RESULTS:The analyses of the sequential serum samples from ZIKV infected individuals showed NS1 specific Abs appeared 2 weeks later than E specific Abs. Notably, human sera from ZIKV infected individuals did not contain cross-reactivity to NS1 proteins of any of the four DENV serotypes. Furthermore, four out of five NS1-specific monoclonal antibodies (mAbs) isolated from ZIKV infected individuals did not bind to DENV NS1 proteins. Only limited amount of cross-reactivity to ZIKV NS1 was displayed in 108 DENV1 immune sera at 1:100 dilution. CONCLUSIONS:The high degree of NS1-specific Abs in both ZIKV and DENV infection revealed here suggest that NS1-based diagnostics would significantly improve the differential diagnosis between DENV and ZIKV infections.


PROVIDER: S-EPMC6000977 | BioStudies | 2018-01-01


REPOSITORIES: biostudies

Similar Datasets

| S-EPMC6901494 | BioStudies
2019-01-01 | S-EPMC6694664 | BioStudies
1000-01-01 | S-EPMC5599479 | BioStudies
2019-01-01 | S-EPMC6938527 | BioStudies
| S-EPMC6289764 | BioStudies
2020-01-01 | S-EPMC7035413 | BioStudies
2019-01-01 | S-EPMC6425182 | BioStudies
1000-01-01 | S-EPMC5850648 | BioStudies
2017-01-01 | S-EPMC5547631 | BioStudies
2016-01-01 | S-EPMC4948328 | BioStudies