Unknown

Dataset Information

0

All-Electronic Quantification of Neuropeptide-Receptor Interaction Using a Bias-Free Functionalized Graphene Microelectrode.


ABSTRACT: Opioid neuropeptides play a significant role in pain perception, appetite regulation, sleep, memory, and learning. Advances in understanding of opioid peptide physiology are held back by the lack of methodologies for real-time quantification of affinities and kinetics of the opioid neuropeptide-receptor interaction at levels typical of endogenous secretion (<50 pM) in biosolutions with physiological ionic strength. To address this challenge, we developed all-electronic opioid-neuropeptide biosensors based on graphene microelectrodes functionalized with a computationally redesigned water-soluble ?-opioid receptor. We used the functionalized microelectrode in a bias-free charge measurement configuration to measure the binding kinetics and equilibrium binding properties of the engineered receptor with [d-Ala2, N-MePhe4, Gly-ol]-enkephalin and ?-endorphin at picomolar levels in real time.

SUBMITTER: Ping J 

PROVIDER: S-EPMC6068397 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

1990-01-01 | S-EPMC1131269 | BioStudies
2015-01-01 | S-EPMC4403092 | BioStudies
1000-01-01 | S-EPMC3482294 | BioStudies
1000-01-01 | S-EPMC6195532 | BioStudies
1997-01-01 | S-EPMC21583 | BioStudies
2019-01-01 | S-EPMC6722706 | BioStudies
1000-01-01 | S-EPMC2705556 | BioStudies
1000-01-01 | S-EPMC47748 | BioStudies
1995-01-01 | S-EPMC1136483 | BioStudies
2019-01-01 | S-EPMC6444270 | BioStudies