Unknown

Dataset Information

0

Molecular Characterization and Geographic Distribution of a Mymonavirus in the Population of Botrytis cinerea.


ABSTRACT: Here, we characterized a negative single-stranded (-ss)RNA mycovirus, Botrytis cinerea mymonavirus 1 (BcMyV1), isolated from the phytopathogenic fungus Botrytis cinerea. The genome of BcMyV1 is 7863 nt in length, possessing three open reading frames (ORF1?3). The ORF1 encodes a large polypeptide containing a conserved mononegaviral RNA-dependent RNA polymerase (RdRp) domain showing homology to the protein L of mymonaviruses, whereas the possible functions of the remaining two ORFs are still unknown. The internal cDNA sequence (10-7829) of BcMyV1 was 97.9% identical to the full-length cDNA sequence of Sclerotinia sclerotiorum negative stranded RNA virus 7 (SsNSRV7), a virus-like contig obtained from Sclerotinia sclerotiorum metatranscriptomes, indicating BcMyV1 should be a strain of SsNSRV7. Phylogenetic analysis based on RdRp domains showed that BcMyV1 was clustered with the viruses in the family Mymonaviridae, suggesting it is a member of Mymonaviridae. BcMyV1 may be widely distributed in regions where B. cinerea occurs in China and even over the world, although it infected only 0.8% of tested B. cinerea strains.

SUBMITTER: Hao F 

PROVIDER: S-EPMC6116283 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

2015-01-01 | S-EPMC4357927 | BioStudies
2014-01-01 | S-EPMC4136314 | BioStudies
2015-01-01 | S-EPMC4452915 | BioStudies
2018-01-01 | S-EPMC5977247 | BioStudies
2019-01-01 | S-EPMC6626909 | BioStudies
2014-01-01 | S-EPMC4143027 | BioStudies
2016-01-01 | S-EPMC4895950 | BioStudies
2012-01-01 | S-EPMC6638627 | BioStudies
2018-01-01 | S-EPMC5943259 | BioStudies
2015-01-01 | S-EPMC4480369 | BioStudies