Unknown

Dataset Information

0

Quantitative Proteomic Analysis of 2D and 3D Cultured Colorectal Cancer Cells: Profiling of Tankyrase Inhibitor XAV939-Induced Proteome.


ABSTRACT: Recently there has been a growing interest in three-dimensional (3D) cell culture systems for drug discovery and development. These 3D culture systems better represent the in vivo cellular environment compared to two-dimensional (2D) cell culture, thereby providing more physiologically reliable information on drug screening and testing. Here we present the quantitative profiling of a drug-induced proteome in 2D- and 3D-cultured colorectal cancer SW480 cells using 2D nanoflow liquid chromatography-tandem mass spectrometry (2D-nLC-MS/MS) integrated with isobaric tags for relative and absolute quantitation (iTRAQ). We identified a total of 4854 shared proteins between 2D- and 3D-cultured SW480 cells and 136/247 differentially expressed proteins (up/down-regulated in 3D compared to 2D). These up/down-regulated proteins were mainly involved in energy metabolism, cell growth, and cell-cell interactions. We also investigated the XAV939 (tankyrase inhibitor)-induced proteome to reveal factors involved in the 3D culture-selective growth inhibitory effect of XAV939 on SW480 cells. We identified novel XAV939-induced proteins, including gelsolin (a possible tumor suppressor) and lactate dehydrogenase A (a key enzyme of glycolysis), which were differentially expressed between 2D- and 3D-cultured SW480 cells. These results provide a promising informative protein dataset to determine the effect of XAV939 on the expression levels of proteins involved in SW480 cell growth.

SUBMITTER: Kim YE 

PROVIDER: S-EPMC6125324 | BioStudies | 2018-01-01

SECONDARY ACCESSION(S): O43913

REPOSITORIES: biostudies

Similar Datasets

2019-01-01 | S-EPMC6781734 | BioStudies
1000-01-01 | S-EPMC4943469 | BioStudies
2019-01-01 | S-EPMC6507402 | BioStudies
2019-01-01 | S-EPMC6856061 | BioStudies
2016-01-01 | S-EPMC5216757 | BioStudies
2019-01-01 | S-EPMC6721645 | BioStudies
1000-01-01 | S-EPMC6121648 | BioStudies
2019-01-01 | S-EPMC6900950 | BioStudies
1000-01-01 | S-EPMC5302961 | BioStudies
2018-01-01 | S-EPMC6171656 | BioStudies