Unknown

Dataset Information

0

Local Oxidative Damage in the Soma and Dendrites Quarantines Neuronal Mitochondria at the Site of Insult.


ABSTRACT: Neurons are highly dependent on mitochondria, but little is known about how they react to a local mitochondrial oxidative insult. We therefore developed a protocol in primary hippocampal cultures that combines the photosensitizer mito-KillerRed with fluorescent biosensors and photoactivatable GFP. We found in both the soma and dendrites that neurons restrict the local increase in mitochondria-derived reactive oxygen species and the decrease in ATP production to the damaged compartment, by quarantining mitochondria. Although the cytosol of both the soma and dendrites became oxidized after mito-KillerRed activation, dendrites were more sensitive to the oxidative insult. Importantly, the impaired mitochondria exhibited decreased motility and fusion, thereby avoiding the spread of oxidation throughout the neuron. These results establish how neurons manage oxidative damage and increase our understanding about the somatodendritic regulation of mitochondrial functions after a local oxidative insult.

SUBMITTER: Grimm A 

PROVIDER: S-EPMC6137705 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

2020-01-01 | S-EPMC7285523 | BioStudies
2019-01-01 | S-EPMC6744920 | BioStudies
2014-07-30 | E-GEOD-58343 | ArrayExpress
| GSE58343 | GEO
2014-10-14 | E-GEOD-61084 | ArrayExpress
2014-10-14 | E-GEOD-61085 | ArrayExpress
2013-01-01 | S-EPMC3877846 | BioStudies
1000-01-01 | S-EPMC4151150 | BioStudies
2019-01-07 | GSE115480 | GEO
2012-01-01 | S-EPMC3489731 | BioStudies