Unknown

Dataset Information

0

Transcriptomic analysis of longitudinal Burkholderia pseudomallei infecting the cystic fibrosis lung.


ABSTRACT: The melioidosis bacterium, Burkholderia pseudomallei, is increasingly being recognised as a pathogen in patients with cystic fibrosis (CF). We have recently catalogued genome-wide variation of paired, isogenic B. pseudomallei isolates from seven Australasian CF cases, which were collected between 4 and 55?months apart. Here, we extend this investigation by documenting the transcriptomic changes in B. pseudomallei in five cases. Following growth in an artificial CF sputum medium, four of the five paired isolates exhibited significant differential gene expression (DE) that affected between 32 and 792 genes. The greatest number of DE events was observed between the strains from patient CF9, consistent with the hypermutator status of the latter strain, which is deficient in the DNA mismatch repair protein MutS. Two patient isolates harboured duplications that concomitantly increased expression of the ?-lactamase-encoding gene penA, and a 35?kb deletion in another abolished expression of 29 genes. Convergent expression profiles in the chronically-adapted isolates identified two significantly downregulated and 17 significantly upregulated loci, including the resistance-nodulation-division (RND) efflux pump BpeEF-OprC, the quorum-sensing hhqABCDE operon, and a cyanide- and pyocyanin-insensitive cytochrome bd quinol oxidase. These convergent pathoadaptations lead to increased expression of pathways that may suppress competing bacterial and fungal pathogens, and that enhance survival in oxygen-restricted environments, the latter of which may render conventional antibiotics less effective in vivo. Treating chronically adapted B. pseudomallei infections with antibiotics designed to target anaerobic infections, such as the nitroimidazole class of antibiotics, may significantly improve pathogen eradication attempts by exploiting this Achilles heel.

SUBMITTER: Price EP 

PROVIDER: S-EPMC6159556 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5587915 | BioStudies
2018-01-01 | S-EPMC6230764 | BioStudies
2013-01-01 | S-EPMC3754293 | BioStudies
2017-01-01 | S-EPMC5388805 | BioStudies
2018-01-01 | S-EPMC6190177 | BioStudies
1000-01-01 | S-EPMC3430303 | BioStudies
2013-01-01 | S-EPMC3719646 | BioStudies
2019-01-01 | S-EPMC6761493 | BioStudies
2015-01-01 | S-EPMC4400425 | BioStudies
2018-01-01 | S-EPMC6201108 | BioStudies