Unknown

Dataset Information

0

The Healing Effect of Human Milk Fat Globule-EGF Factor 8 Protein (MFG-E8) in A Rat Model of Parkinson's Disease.


ABSTRACT: We searched for drugs that alleviate the reduction of dopaminergic neurons caused by the administration of lipopolysaccharide (LPS) to the substantia nigra of the rat brain. Human milk fat globule-EGF factor 8 protein (MFG-E8) is similar to MFG-E8-S, a short isoform, of the mouse MFG-E8. However, the function of MFG-E8-S was not clear. Rats with LPS-induced Parkinson's disease were prepared and the effects of human MFG-E8 were examined. MFG-E8 improved the significant reduction in mesencephalic dopamine neurons induced by the administration of LPS. LPS was administered to human induced pluripotent stem cell (iPSC)-derived dopaminergic neurons to induce inflammation and the effect of MFG-E8 was examined. MFG-E8 showed no toxicity toward neurons. We reanalyzed the results using public microarray data. MFG-E8 mRNA was found to be expressed in all parts of the body, particularly by adipose-derived stem cells (ADSCs). Furthermore, we investigated the culture supernatant of ADSCs using the liquid chromatography-tandem mass spectrometry (LC?MS/MS) analysis method and successfully identified the peptide of the MFG-E8 F5/8 type C domain. The results suggested that MFG-E8-S may have a preventive effect against Parkinson's disease.

SUBMITTER: Nakashima Y 

PROVIDER: S-EPMC6162645 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

2011-01-01 | S-EPMC3184509 | BioStudies
2019-01-01 | S-EPMC6823494 | BioStudies
1000-01-01 | S-EPMC4148880 | BioStudies
2011-01-01 | S-EPMC3217009 | BioStudies
1000-01-01 | S-EPMC4990839 | BioStudies
2014-01-01 | S-EPMC4076467 | BioStudies
2015-01-01 | S-EPMC4515172 | BioStudies
1000-01-01 | S-EPMC5307389 | BioStudies
2015-01-01 | S-EPMC4525298 | BioStudies
2016-07-21 | E-GEOD-73229 | ArrayExpress