Unknown

Dataset Information

0

Near-infrared STED nanoscopy with an engineered bacterial phytochrome.


ABSTRACT: The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650?nm and the onset of increased water absorption at 900?nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.

SUBMITTER: Kamper M 

PROVIDER: S-EPMC6232180 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC4479004 | BioStudies
2013-01-01 | S-EPMC3548795 | BioStudies
1000-01-01 | S-EPMC4879624 | BioStudies
2013-01-01 | S-EPMC3637247 | BioStudies
1000-01-01 | S-EPMC5394456 | BioStudies
2009-01-01 | S-EPMC2957894 | BioStudies
2019-01-01 | S-EPMC6712041 | BioStudies
2017-01-01 | S-EPMC5595794 | BioStudies
1000-01-01 | S-EPMC5807511 | BioStudies
2019-01-01 | S-EPMC6423018 | BioStudies