Unknown

Dataset Information

0

The Ric-8A/Gα13/FAK signalling cascade controls focal adhesion formation during neural crest cell migration in Xenopus.


ABSTRACT: Ric-8A is a pleiotropic guanine nucleotide exchange factor involved in the activation of various heterotrimeric G-protein pathways during adulthood and early development. Here, we sought to determine the downstream effectors of Ric-8A during the migration of the vertebrate cranial neural crest (NC) cells. We show that the Gα13 knockdown phenocopies the Ric-8A morphant condition, causing actin cytoskeleton alteration, protrusion instability, and a strong reduction in the number and dynamics of focal adhesions. In addition, the overexpression of Gα13 is sufficient to rescue Ric-8A-depleted cells. Ric-8A and Gα13 physically interact and colocalize in protrusions of the cells leading edge. The focal adhesion kinase FAK colocalizes and interacts with the endogenous Gα13, and a constitutively active form of Src efficiently rescues the Gα13 morphant phenotype in NC cells. We propose that Ric-8A-mediated Gα13 signalling is required for proper cranial NC cell migration by regulating focal adhesion dynamics and protrusion formation.

SUBMITTER: Toro-Tapia G 

PROVIDER: S-EPMC6262797 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

Similar Datasets

2015-01-01 | S-EPMC4831272 | BioStudies
1000-01-01 | S-EPMC2516996 | BioStudies
2020-01-01 | S-EPMC7044438 | BioStudies
2016-01-01 | S-EPMC5182059 | BioStudies
2016-01-01 | S-EPMC4945744 | BioStudies
2018-01-01 | S-EPMC6526509 | BioStudies
2010-01-01 | S-EPMC2897540 | BioStudies
2014-01-01 | S-EPMC3897744 | BioStudies
2016-01-01 | S-EPMC5002080 | BioStudies
2015-01-01 | S-EPMC4321267 | BioStudies