Dataset Information


Article expression, purification, and characterization of Cu/ZnSOD from Panax ginseng.

ABSTRACT: Superoxide dismutase (SOD) has a strong antioxidant effect, but the traditional SOD extraction method is not the most efficient method of SOD amplification. In this study, we report the cloning of the Cu/ZnSOD gene from Panax ginseng into a temperature-regulated expression plasmid, pBV220. Cu/ZnSOD inclusion bodies were expressed in E. coli at a high level. Then, the inclusion bodies were purified by ion-exchange chromatography and molecular sieve chromatography. Finally, we obtained stable SOD in the bacterial broth, with a protein content of 965 mg/L and enzyme specific activity of 9389.96 U/mg. These results provide a foundation for future studies on the antioxidant mechanisms of ginseng and the development and application of ginseng Cu/ZnSOD.

PROVIDER: S-EPMC6270835 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC4730280 | BioStudies
| S-EPMC8161062 | BioStudies
| S-EPMC6409742 | BioStudies
| S-EPMC4144957 | BioStudies
| S-EPMC8193680 | BioStudies
| S-EPMC2762984 | BioStudies
2003-01-01 | S-EPMC1223582 | BioStudies
2001-01-01 | S-EPMC1222117 | BioStudies
| S-EPMC2234264 | BioStudies
| S-EPMC3098211 | BioStudies