Dataset Information


Correlative iPALM and SEM resolves virus cavity and Gag lattice defects in HIV virions.

ABSTRACT: Interferometric Photo-Activation-Localization-Microscopy (iPALM) localizes single fluorescent molecules with 20 nm lateral and 10 nm axial resolution. We present a method utilizing glass coverslip lithography for correlative imaging between iPALM and scanning electron microscopy (SEM). Using iPALM on HIV Gag-Dendra virus-like particles (VLPs) we localized the position of HIV Gag proteins. Based on these localizations we reconstructed the central cavity of the VLPs along with imperfections within the HIV Gag lattice. The SEM images and iPALM images overlap and show imaging from single VLPs immobilized on glass coverslips. The localization of many HIV proteins including accessory proteins and Gag-Pol remains unknown, we discuss how the specificity of iPALM coupled with SEM has the potential for resolving more of HIV proteins.

SUBMITTER: Pedersen M 

PROVIDER: S-EPMC6330563 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

Similar Datasets

2020-01-01 | S-EPMC7399489 | BioStudies
1000-01-01 | S-EPMC1266096 | BioStudies
2011-01-01 | S-EPMC3227654 | BioStudies
1000-01-01 | S-EPMC2717261 | BioStudies
2016-01-01 | S-EPMC4795674 | BioStudies
1000-01-01 | S-EPMC4513081 | BioStudies
1000-01-01 | S-EPMC4097806 | BioStudies
2018-01-01 | S-EPMC6295721 | BioStudies
1000-01-01 | S-EPMC3838742 | BioStudies
2016-01-01 | S-EPMC5008088 | BioStudies