Unknown

Dataset Information

0

KCC2-Mediated Cl- Extrusion Modulates Spontaneous Hippocampal Network Events in Perinatal Rats and Mice.


ABSTRACT: It is generally thought that hippocampal neurons of perinatal rats and mice lack transport-functional K-Cl cotransporter KCC2, and that Cl- regulation is dominated by Cl- uptake via the Na-K-2Cl cotransporter NKCC1. Here, we demonstrate a robust enhancement of spontaneous hippocampal network events (giant depolarizing potentials [GDPs]) by the KCC2 inhibitor VU0463271 in neonatal rats and late-gestation, wild-type mouse embryos, but not in their KCC2-null littermates. VU0463271 increased the depolarizing GABAergic synaptic drive onto neonatal CA3 pyramidal neurons, increasing their spiking probability and synchrony during the rising phase of a GDP. Our data indicate that Cl- extrusion by KCC2 is involved in modulation of GDPs already at their developmental onset during the perinatal period in mice and rats.

SUBMITTER: Spoljaric I 

PROVIDER: S-EPMC6352714 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC5224924 | BioStudies
2003-01-01 | S-EPMC6741087 | BioStudies
2016-01-01 | S-EPMC5003660 | BioStudies
2018-01-01 | S-EPMC6176565 | BioStudies
2013-01-01 | S-EPMC3587235 | BioStudies
2017-01-01 | S-EPMC5640428 | BioStudies
2000-01-01 | S-EPMC6772739 | BioStudies
2017-10-16 | PXD006046 | Pride
2009-01-01 | S-EPMC2788850 | BioStudies
2014-01-01 | S-EPMC6340141 | BioStudies