Dataset Information


Promoter-proximal pausing mediated by the exon junction complex regulates splicing.

ABSTRACT: Promoter-proximal pausing of RNA polymerase II (Pol II) is a widespread transcriptional regulatory step across metazoans. Here we find that the nuclear exon junction complex (pre-EJC) is a critical and conserved regulator of this process. Depletion of pre-EJC subunits leads to a global decrease in Pol II pausing and to premature entry into elongation. This effect occurs, at least in part, via non-canonical recruitment of pre-EJC components at promoters. Failure to recruit the pre-EJC at promoters results in increased binding of the positive transcription elongation complex (P-TEFb) and in enhanced Pol II release. Notably, restoring pausing is sufficient to rescue exon skipping and the photoreceptor differentiation defect associated with depletion of pre-EJC components in vivo. We propose that the pre-EJC serves as an early transcriptional checkpoint to prevent premature entry into elongation, ensuring proper recruitment of RNA processing components that are necessary for exon definition.


PROVIDER: S-EPMC6355915 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

Similar Datasets

2019-01-22 | GSE92379 | GEO
2019-01-22 | GSE120880 | GEO
2019-01-22 | GSE110671 | GEO
2019-01-22 | GSE106904 | GEO
2019-01-22 | GSE113278 | GEO
2019-01-22 | GSE106909 | GEO
2019-01-22 | GSE92389 | GEO
2019-01-22 | GSE92493 | GEO
2019-01-22 | GSE92388 | GEO
2019-01-22 | GSE106907 | GEO