Unknown

Dataset Information

0

The rsmS (ybaM) mutation causes bypass suppression of the RsmAB post-transcriptional virulence regulation system in enterobacterial phytopathogens.


ABSTRACT: Plant cell wall degrading enzymes (PCWDEs) are the primary virulence determinants of soft rotting bacteria such as the potato pathogen, Pectobacterium atrosepticum. The regulation of secondary metabolite (Rsm) system controls production of PCWDEs in response to changing nutrient conditions. This work identified a new suppressor of an rsmB mutation - ECA1172 or rsmS (rsmB suppressor). Mutants defective in rsmB (encoding a small regulatory RNA), show reduced elaboration of the quorum sensing molecule (N-3-oxohexanoyl-homoserine lactone; OHHL) and PCWDEs. However, OHHL and PCWDE production were partially restored in an rsmB, rsmS double mutant. Single rsmS mutants, overproduced PCWDEs and OHHL relative to wild type P. atrosepticum and exhibited hypervirulence in potato. RsmS overproduction also resulted in increased PCWDEs and OHHL. Homology searches revealed rsmS conservation across pathogens such as Escherichia coli (ybaM), Dickeya solani, Klebsiella pneumoniae and Shigella flexneri. An rsmS mutant of Pectobacterium carotovorum ATCC39048 showed bypass of rsmB-dependent repression of PCWDEs and OHHL production. P. carotovorum ATCC39048 produces the ?-lactam antibiotic, 1-carbapen-2-em-3-carboxylic acid (a carbapenem). Production of the antibiotic was repressed in an rsmB mutant but partially restored in an rsmB, rsmS double mutant. This work highlights the importance of RsmS, as a conserved pleiotropic regulator of virulence and antibiotic biosynthesis.

SUBMITTER: Monson RE 

PROVIDER: S-EPMC6418279 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

Similar Datasets

2012-11-02 | E-MTAB-1167 | BioStudies
2012-11-02 | E-MTAB-1167 | ArrayExpress
1000-01-01 | S-EPMC4260167 | BioStudies
2020-01-01 | S-EPMC7117878 | BioStudies
2013-01-01 | S-EPMC3765535 | BioStudies
2016-01-01 | S-EPMC4814525 | BioStudies
2019-01-01 | S-EPMC6616942 | BioStudies
1000-01-01 | S-EPMC4622587 | BioStudies
2012-01-01 | S-EPMC3302902 | BioStudies
2018-01-01 | S-EPMC6286560 | BioStudies