Dataset Information


Core Fucosylation of Maternal Milk N-Glycan Evokes B Cell Activation by Selectively Promoting the l-Fucose Metabolism of Gut Bifidobacterium spp. and Lactobacillus spp.

ABSTRACT: The maternal milk glycobiome is crucial for shaping the gut microbiota of infants. Although high core fucosylation catalyzed by fucosyltransferase 8 (Fut8) is a general feature of human milk glycoproteins, its role in the formation of a healthy microbiota has not been evaluated. In this study, we found that the core-fucosylated N-glycans in milk of Chinese mothers selectively promoted the colonization of specific gut microbial groups, such as Bifidobacterium spp. and Lactobacillus spp. in their breast-fed infants during lactation. Compared with Fut8+/+ (WT) mouse-fed neonates, the offspring fed by Fut8 +/- maternal mice had a distinct gut microbial profile, which was featured by a significant reduction of Lactobacillus spp., Bacteroides spp., and Bifidobacterium spp. and increased abundance of members of the Lachnospiraceae NK4A136 group and Akkermansia spp. Moreover, these offspring mice showed a lower proportion of splenic CD19+ CD69+ B lymphocytes and attenuated humoral immune responses upon ovalbumin (OVA) immunization. In vitro studies demonstrated that the chemically synthesized core-fucosylated oligosaccharides possessed the ability to promote the growth of tested Bifidobacterium and Lactobacillus strains in minimal medium. The resulting L-fucose metabolites, lactate and 1,2-propanediol, could promote the activation of B cells via the B cell receptor (BCR)-mediated signaling pathway.IMPORTANCE This study provides novel evidence for the critical role of maternal milk protein glycosylation in shaping early-life gut microbiota and promoting B cell activation of neonates. The special core-fucosylated oligosaccharides might be promising prebiotics for the personalized nutrition of infants.


PROVIDER: S-EPMC6445936 | BioStudies | 2019-01-01


REPOSITORIES: biostudies

Similar Datasets

2018-01-01 | S-EPMC6306508 | BioStudies
2020-01-01 | S-EPMC7266941 | BioStudies
1000-01-01 | S-EPMC3796377 | BioStudies
2019-01-01 | S-EPMC6650860 | BioStudies
2020-01-01 | S-EPMC7453906 | BioStudies
2013-01-01 | S-EPMC3841168 | BioStudies
2015-01-01 | S-EPMC4412032 | BioStudies
1000-01-01 | S-EPMC5240090 | BioStudies
2012-01-01 | S-EPMC3293811 | BioStudies
2012-01-01 | S-EPMC3272021 | BioStudies