Dataset Information


Enhanced secretion of human ?1-antitrypsin expressed with a novel glycosylation module in tobacco BY-2 cell culture.

ABSTRACT: Expression of recombinant proteins fused to a novel glycomodule tag, termed hydroxyproline (Hyp)-O-glycosylated peptides (HypGP), was earlier found to boost secreted protein yields up to 500-fold in plant cell culture. Here, this technology was applied to the expression of human protease inhibitor ?1-antitrypsin (AAT) in tobacco BY-2 cell culture. A designer HypGP tag composed of a 'Ala-Pro' motif of 20 units, or (AP)20, was engineered either at the N- or C-terminal end of AAT. The (AP)20 tag substantially increased the secreted yields of the recombinant AAT up to 34.7 mg/L. However, the (AP)20-tagged AAT products were frequently subjected to proteolytic processing. The intact AAT-(AP)20 along with some of the truncated AAT domains exhibited desired biological activity in inhibiting elastase. The results from this research demonstrated that the designer (AP)20 module engineered in BY-2 cells could function as a molecular carrier to substantially enhance the secreted yields of the recombinant AAT.


PROVIDER: S-EPMC6527068 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

Similar Datasets

2014-01-01 | S-EPMC4215913 | BioStudies
2019-01-01 | S-EPMC6383191 | BioStudies
2012-01-01 | S-EPMC3443621 | BioStudies
2015-01-01 | S-EPMC4669469 | BioStudies
1000-01-01 | S-EPMC4364503 | BioStudies
2012-01-01 | S-EPMC3368911 | BioStudies
2018-01-01 | S-EPMC6044564 | BioStudies
2019-01-01 | S-EPMC6792240 | BioStudies
2013-01-01 | S-EPMC3720964 | BioStudies
2019-01-01 | S-EPMC6682001 | BioStudies