Unknown

Dataset Information

0

Mitochondrial genomics of human pathogenic parasite Leishmania (Viannia) panamensis.


ABSTRACT: Background:The human parasite Leishmania (V.) panamensis is one of the pathogenic species responsible for cutaneous leishmaniasis in Central and South America. Despite its importance in molecular parasitology, its mitochondrial genome, divided into minicircles and maxicircles, haven't been described so far. Methods:Using NGS-based sequencing (454 and ILLUMINA), and combining de novo genome assembly and mapping strategies, we report the maxicircle kDNA annotated genome of L. (V.) panamensis, the first reference of this molecule for the subgenus Viannia. A comparative genomics approach is performed against other Leishmania and Trypanosoma species. Results:The results show synteny of mitochondrial genes of L. (V.) panamensis with other kinetoplastids. It was also possible to identify nucleotide variants within the coding regions of the maxicircle, shared among some of them and others specific to each strain. Furthermore, we compared the minicircles kDNA sequences of two strains and the results show that the conserved and divergent regions of the minicircles exhibit strain-specific associations.

SUBMITTER: Urrea DA 

PROVIDER: S-EPMC6611448 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

Similar Datasets

2019-01-01 | S-EPMC6826401 | BioStudies
2009-01-01 | S-EPMC2763077 | BioStudies
2020-01-01 | S-EPMC7140786 | BioStudies
1000-01-01 | S-EPMC3697465 | BioStudies
1000-01-01 | S-EPMC5963845 | BioStudies
2008-01-01 | S-EPMC2538871 | BioStudies
1000-01-01 | S-EPMC4337576 | BioStudies
2005-01-01 | S-EPMC1087824 | BioStudies
2014-01-01 | S-EPMC4135736 | BioStudies
1000-01-01 | S-EPMC1170432 | BioStudies