Dataset Information


Role of the Srs2-Rad51 Interaction Domain in Crossover Control in Saccharomyces cerevisiae.

ABSTRACT: Saccharomyces cerevisiae Srs2, in addition to its well-documented antirecombination activity, has been proposed to play a role in promoting synthesis-dependent strand annealing (SDSA). Here we report the identification and characterization of an SRS2 mutant with a single amino acid substitution (srs2-F891A) that specifically affects the Srs2 pro-SDSA function. This residue is located within the Srs2-Rad51 interaction domain and embedded within a protein sequence resembling a BRC repeat motif. The srs2-F891A mutation leads to a complete loss of interaction with Rad51 as measured through yeast two-hybrid analysis and a partial loss of interaction as determined through protein pull-down assays with purified Srs2, Srs2-F891A, and Rad51 proteins. Even though previous work has shown that internal deletions of the Srs2-Rad51 interaction domain block Srs2 antirecombination activity in vitro, the Srs2-F891A mutant protein, despite its weakened interaction with Rad51, exhibits no measurable defect in antirecombination activity in vitro or in vivo Surprisingly, srs2-F891A shows a robust shift from noncrossover to crossover repair products in a plasmid-based gap repair assay, but not in an ectopic physical recombination assay. Our findings suggest that the Srs2 C-terminal Rad51 interaction domain is more complex than previously thought, containing multiple interaction sites with unique effects on Srs2 activity.

PROVIDER: S-EPMC6707447 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC3791737 | BioStudies
| S-EPMC3213327 | BioStudies
| S-EPMC5441872 | BioStudies
| S-EPMC3730916 | BioStudies
2012-01-01 | S-EPMC3439891 | BioStudies
| S-EPMC8230603 | BioStudies
| S-EPMC3390654 | BioStudies
| S-EPMC3338270 | BioStudies
| S-EPMC3979297 | BioStudies
| S-EPMC3594751 | BioStudies