Dataset Information


MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus.

ABSTRACT: The sea cucumber (Apostichopus japonicus) has become a good model organism for studying environmentally induced aestivation in marine invertebrates. We hypothesized that mechanisms that arrest energy-expensive cell cycle activity would contribute significantly to establishing the hypometabolic state during aestivation. Cyclin A is a core and particularly interesting cell cycle regulator that functions in both the S phase and in mitosis. In the present study, negative relationships between miR-200-3p and AjCA expressions were detected at both the transcriptional and the translational levels during aestivation in A. japonicus. Dual-luciferase reporter assays confirmed the targeted location of the miR-200-3p binding site within the AjCA gene transcript. Furthermore, gain- and loss-of-function experiments were conducted in vivo with sea cucumbers to verify the interaction between miR-200-3p and AjCA in intestine tissue by qRT-PCR and Western blotting. The results show that the overexpression of miR-200-3p mimics suppressed AjCA transcript levels and translated protein production, whereas transfection with a miR-200-3p inhibitor enhanced both AjCA mRNA and AjCA protein in A. japonicus intestine. Our findings suggested a potential mechanism that reversibly arrests cell cycle progression during aestivation, which may center on miR-200-3p inhibitory control over the translation of cyclin A mRNA transcripts.


PROVIDER: S-EPMC6721757 | BioStudies | 2019-01-01


REPOSITORIES: biostudies

Similar Datasets

2018-01-01 | S-EPMC6173160 | BioStudies
2013-01-01 | S-EPMC3797095 | BioStudies
| GSE48867 | GEO
2014-06-06 | E-GEOD-48867 | ArrayExpress
2014-06-06 | E-GEOD-48868 | ArrayExpress
| GSE52225 | GEO
2018-01-01 | S-EPMC6018497 | BioStudies
2014-10-01 | E-GEOD-52225 | ArrayExpress
| GSE53815 | GEO
2014-10-01 | E-GEOD-52224 | ArrayExpress