Unknown

Dataset Information

0

The NKCC1 antagonist bumetanide mitigates interneuronopathy associated with ethanol exposure in utero.


ABSTRACT: Prenatal exposure to ethanol induces aberrant tangential migration of corticopetal GABAergic interneurons, and long-term alterations in the form and function of the prefrontal cortex. We have hypothesized that interneuronopathy contributes significantly to the pathoetiology of fetal alcohol spectrum disorders (FASD). Activity-dependent tangential migration of GABAergic cortical neurons is driven by depolarizing responses to ambient GABA present in the cortical enclave. We found that ethanol exposure potentiates the depolarizing action of GABA in GABAergic cortical interneurons of the embryonic mouse brain. Pharmacological antagonism of the cotransporter NKCC1 mitigated ethanol-induced potentiation of GABA depolarization and prevented aberrant patterns of tangential migration induced by ethanol in vitro. In a model of FASD, maternal bumetanide treatment prevented interneuronopathy in the prefrontal cortex of ethanol exposed offspring, including deficits in behavioral flexibility. These findings position interneuronopathy as a mechanism of FASD symptomatology, and posit NKCC1 as a pharmacological target for the management of FASD.

SUBMITTER: Skorput AG 

PROVIDER: S-EPMC6768659 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC6596671 | BioStudies
2020-01-01 | S-EPMC7706035 | BioStudies
2018-01-01 | S-EPMC6141474 | BioStudies
2014-01-01 | S-EPMC4085902 | BioStudies
1000-01-01 | S-EPMC3059882 | BioStudies
2009-01-01 | S-EPMC2910524 | BioStudies
1000-01-01 | S-EPMC6104028 | BioStudies
2017-01-01 | S-EPMC5650248 | BioStudies
2012-01-01 | S-EPMC3390258 | BioStudies
2002-01-01 | S-EPMC138601 | BioStudies