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Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation.


ABSTRACT: Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany "Filter Aided Sample Preparation - A Tutorial" (Wi?niewski, 2019).

SUBMITTER: Wisniewski JR 

PROVIDER: S-EPMC6811893 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

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