Unknown

Dataset Information

0

MicroRNA-221 restricts human cytomegalovirus replication via promoting type I IFN production by targeting SOCS1/NF-?B pathway.


ABSTRACT: HCMV is a common pathogen for human with relatively high prevalence, which could be life-threatened in immunodeficient patients and lead to significant birth defects in newborns. In this study, we firstly report that HCMV infection significantly enhances the expression of microRNA-221 (miR-221) in Neural Precursor Cells (NPCs). We found that miR-221 directly targets at the 3'-UTR of suppressor of cytokine signaling 1 (SOCS1) and suppresses SOCS1 expression at the both mRNA and protein levels. MiR-221 overexpression restrained HCMV replication by promoting type I interferon (IFN) and interferon stimulating genes (ISGs) production, whereas reintroduction of SOCS1 abrogated the miR-221-induced effects on HCMV replication. Importantly, miR-221 positively regulated the phosphorylation and activation of NF-?B by suppressing SOCS1. What's more, miR-221 agomir alleviated MCMV-induced tissue injury by promoting type I IFN antiviral activities in vivo. Thus, miR-221 modulates the infection and replication of HCMV as an intrinsic antiviral factor, and could be developed as a treatment target for anti-HCMV treatment.

PROVIDER: S-EPMC6816349 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC7256192 | BioStudies
| S-EPMC6206482 | BioStudies
| S-EPMC7688008 | BioStudies
| S-EPMC4628668 | BioStudies
| S-EPMC6093694 | BioStudies
| S-EPMC7162240 | BioStudies
| S-EPMC5573403 | BioStudies
| S-EPMC5845882 | BioStudies
| S-EPMC4097622 | BioStudies
| S-EPMC4817095 | BioStudies