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CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci.


ABSTRACT: Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacons (MBs) that can undergo fluorescence resonance energy transfer (FRET) and demonstrated that the resulting system, CRISPR/dual-FRET MB, enables dynamic imaging of non-repetitive genomic loci with only three unique sgRNAs.

SUBMITTER: Mao S 

PROVIDER: S-EPMC6847002 | BioStudies | 2019-01-01

REPOSITORIES: biostudies

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