Unknown

Dataset Information

0

Dissecting promoter of InMYB1 gene showing petal-specific expression.


ABSTRACT: We had previously reported that the InMYB1 promoter, the 1023 bp upstream region of InMYB1, works petal-specifically in various dicot plants by recognizing petal identity at a cellular level. To determine the petal-specific region in the InMYB1 promoter, Arabidopsis plants harboring InMYB1_1023b::GUS (?-glucuronidase), InMYB1_713b::GUS, InMYB1_506b::GUS, InMYB1_403b::GUS, InMYB1_332b::GUS, InMYB1_200b::GUS and InMYB1_140b::GUS were produced and confirmed a shortest region, which has the petal-specific promoter activity by using histochemical GUS assay. Petal-specific GUS staining was not observed in the Arabidopsis plants transformed with InMYB1_200b::GUS and InMYB1_140b::GUS, but observed in transgenic Arabidopsis plants harboring from InMYB1_1023b::GUS to InMYB1_332b::GUS. cDNA sequence of InMYB1 shows that 120 bp upstream region of InMYB1 is 5' untranslated region, suggesting that the 332-121 bp upstream region of InMYB1 contains an important element for petal-specific gene expression. In the Arabidopsis harboring the InMYB1_332-121b×3_TATA_?::GUS, petal-specific GUS staining was observed and the staining was stronger than in the Arabidopsis harboring InMYB1_1023b::GUS. This result shows that the 332-121 bp region is enough and essential for the petal specificity and the InMYB1_332-121b×3_TATA_? could be used for the molecular breeding of floricultural crops.

SUBMITTER: Azuma M 

PROVIDER: S-EPMC6879369 | BioStudies | 2018-01-01

SECONDARY ACCESSION(S): AB232773

REPOSITORIES: biostudies

Similar Datasets

2016-01-01 | S-EPMC4882030 | BioStudies
1000-01-01 | S-EPMC2844064 | BioStudies
2016-01-01 | S-EPMC5183650 | BioStudies
2012-01-01 | S-EPMC3254680 | BioStudies
2020-01-01 | S-EPMC7072871 | BioStudies
2015-01-01 | S-EPMC4854347 | BioStudies
2019-01-01 | S-EPMC6651508 | BioStudies
1000-01-01 | S-EPMC3350915 | BioStudies
2018-01-01 | S-EPMC5811034 | BioStudies
2013-01-01 | S-EPMC3876083 | BioStudies