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Ajudecumin A from Ajuga ovalifolia var. calantha exhibits anti-inflammatory activity in lipopolysaccharide-activated RAW264.7 murine macrophages and animal models of acute inflammation.

ABSTRACT: CONTEXT:Ajuga ovalifolia Bur. et Franch. var. calantha (Diels) C. Y. Wu et C. Chen (Labiatae), a traditional Chinese medicine, has been used to treat several inflammatory diseases. OBJECTIVE:To assess the anti-inflammatory activity of ajudecumin A isolated from Ajuga ovalifolia var. calantha, and its possible mechanisms. MATERIALS AND METHODS:Lipopolysaccharide (LPS, 0.5??g/mL)-stimulated RAW264.7 macrophages were used to assess the anti-inflammatory activity of ajudecumin A (1-40??M) in vitro. Nitric oxide levels were evaluated by Griess reagent. The mRNA levels of iNOS, COX-2, TNF-?, IL-1? and IL-6 were determined using qRT-PCR. Phosphorylation of ERK, JNK, p38 MAPK and I?B? were detected by western Blot. To further assess the anti-inflammatory of ajudecumin A in vivo, mice were oral treated with ajudecumin A (10?mg/kg) or dexamethasone (0.25?mg/kg, positive control) for 5?days before administration of carrageenan or xylene. Paw and ear edema were then measured, respectively. RESULTS:Ajudecumin A (10-40??M) decreased LPS-induced nitric oxide production with an IC50 value of 16.19??M. Ajudecumin A (20 and 40??M) also attenuated cell spreading and formation of pseudopodia-like structures, and decreased the mRNA levels of iNOS (55.23-67.04%, p?


PROVIDER: S-EPMC7011979 | BioStudies | 2018-01-01

REPOSITORIES: biostudies

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