Dataset Information


Small RNA Profiling of piRNAs in Colorectal Cancer Identifies Consistent Overexpression of piR-24000 That Correlates Clinically with an Aggressive Disease Phenotype.

ABSTRACT: Piwi-interacting RNAs (piRNAs) represent a novel class of small non-coding RNAs (ncRNAs) that have been shown to have a deregulated expression in several cancers, although their clinical significance in colorectal cancer (CRC) remains unclear. With an aim of delineating the piRNA distribution in CRC, we conducted a systematic discovery and validation of piRNAs within two clinical cohorts. In the discovery phase, we profiled tumor and adjacent normal tissues from 18 CRC patients by deep sequencing and identified a global piRNA downregulation in CRC. Moreover, we identified piR-24000 as an unexplored piRNA that was significantly overexpressed in CRC. Using qPCR, we validated the overexpression of piR-24000 in 87 CRC patients. Additionally, we identified a significant association between a high expression of piR-24000 and an aggressive CRC phenotype including poor differentiation, presence of distant metastases, and a higher stage. Lastly, ROC analysis demonstrated a strong diagnostic power of piR-24000 in discriminating CRC patients from normal subjects. Taken together, this study provides one of the earliest large-scale reports of the global distribution of piRNAs in CRC. In addition, piR-24000 was identified as a likely oncogene in CRC that can serve as a biomarker or a therapeutic target.

PROVIDER: S-EPMC7016796 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC7288952 | BioStudies
| S-EPMC5581525 | BioStudies
| S-EPMC8415021 | BioStudies
| S-EPMC4534471 | BioStudies
| S-EPMC5791351 | BioStudies
| S-EPMC8605029 | BioStudies
| S-EPMC8447905 | BioStudies
| S-EPMC6340885 | BioStudies
| S-EPMC5470966 | BioStudies
| S-EPMC4557300 | BioStudies