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Electrical stimulation of renal nerves for modulating urine glucose excretion in rats.


ABSTRACT: Background:The role of the kidney in glucose homeostasis has gained global interest. Kidneys are innervated by renal nerves, and renal denervation animal models have shown improved glucose regulation. We hypothesized that stimulation of renal nerves at kilohertz frequencies, which can block propagation of action potentials, would increase urine glucose excretion. Conversely, we hypothesized that low frequency stimulation, which has been shown to increase renal nerve activity, would decrease urine glucose excretion. Methods:We performed non-survival experiments on male rats under thiobutabarbital anesthesia. A cuff electrode was placed around the left renal artery, encircling the renal nerves. Ureters were cannulated bilaterally to obtain urine samples from each kidney independently for comparison. Renal nerves were stimulated at kilohertz frequencies (1-50 kHz) or low frequencies (2-5 Hz), with intravenous administration of a glucose bolus shortly into the 25-40-min stimulation period. Urine samples were collected at 5-10-min intervals, and colorimetric assays were used to quantify glucose excretion and concentration between stimulated and non-stimulated kidneys. A Kruskal-Wallis test was performed across all stimulation frequencies (??=?0.05), followed by a post-hoc Wilcoxon rank sum test with Bonferroni correction (??=?0.005). Results:For kilohertz frequency trials, the stimulated kidney yielded a higher average total urine glucose excretion at 33 kHz (+?24.5%; n?=?9) than 1 kHz (-?5.9%; n?=?6) and 50 kHz (+?2.3%; n?=?14). In low frequency stimulation trials, 5 Hz stimulation led to a lower average total urine glucose excretion (-?40.4%; n?=?6) than 2 Hz (-?27.2%; n?=?5). The average total urine glucose excretion between 33 kHz and 5 Hz was statistically significant (p?

PROVIDER: S-EPMC7098252 | BioStudies |

REPOSITORIES: biostudies

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