Dataset Information


Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes.

ABSTRACT: Primary adult cardiomyocyte (aCM) represent the mature form of myocytes found in the adult heart. However, culture of aCMs in particular is challenged by poor survival and loss of phenotype, rendering extended in vitro experiments unfeasible. Here, we establish murine aCM culture methods that enhance survival and maintain sarcomeric structure and Ca2+ cycling to enable physiologically relevant contractile force measurements. We also demonstrate genetic and small-molecule manipulations that probe mechanisms underlying myocyte functional performance. Together, these refinements to aCM culture present a toolbox with which to advance our understanding of myocardial physiology.

SUBMITTER: Callaghan NI 

PROVIDER: S-EPMC7214405 | BioStudies | 2020-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5575972 | BioStudies
1000-01-01 | S-EPMC4252891 | BioStudies
2015-01-01 | S-EPMC4677315 | BioStudies
2017-01-01 | S-EPMC6334671 | BioStudies
2018-01-01 | S-EPMC6542357 | BioStudies
2019-01-01 | S-EPMC6950401 | BioStudies
1000-01-01 | S-EPMC5225489 | BioStudies
2020-01-01 | S-EPMC7444578 | BioStudies
2016-03-22 | E-MTAB-3982 | BioStudies
1000-01-01 | S-EPMC3916762 | BioStudies