Dataset Information


A Genome-Wide Screen in Mice To Identify Cell-Extrinsic Regulators of Pulmonary Metastatic Colonisation.

ABSTRACT: Metastatic colonization, whereby a disseminated tumor cell is able to survive and proliferate at a secondary site, involves both tumor cell-intrinsic and -extrinsic factors. To identify tumor cell-extrinsic (microenvironmental) factors that regulate the ability of metastatic tumor cells to effectively colonize a tissue, we performed a genome-wide screen utilizing the experimental metastasis assay on mutant mice. Mutant and wildtype (control) mice were tail vein-dosed with murine metastatic melanoma B16-F10 cells and 10 days later the number of pulmonary metastatic colonies were counted. Of the 1,300 genes/genetic locations (1,344 alleles) assessed in the screen 34 genes were determined to significantly regulate pulmonary metastatic colonization (15 increased and 19 decreased; P < 0.005 and genotype effect <-55 or >+55). While several of these genes have known roles in immune system regulation (Bach2, Cyba, Cybb, Cybc1, Id2, Igh-6, Irf1, Irf7, Ncf1, Ncf2, Ncf4 and Pik3cg) most are involved in a disparate range of biological processes, ranging from ubiquitination (Herc1) to diphthamide synthesis (Dph6) to Rho GTPase-activation (Arhgap30 and Fgd4), with no previous reports of a role in the regulation of metastasis. Thus, we have identified numerous novel regulators of pulmonary metastatic colonization, which may represent potential therapeutic targets.

SUBMITTER: van der Weyden L 

PROVIDER: S-EPMC7263671 | BioStudies | 2020-01-01

REPOSITORIES: biostudies

Similar Datasets

2020-01-01 | S-EPMC7288688 | BioStudies
2018-01-01 | S-EPMC6204876 | BioStudies
2012-01-01 | S-EPMC3349753 | BioStudies
1000-01-01 | S-EPMC6296060 | BioStudies
2021-01-01 | S-EPMC7832311 | BioStudies
1000-01-01 | S-EPMC2990482 | BioStudies
2017-01-01 | S-EPMC5697015 | BioStudies
2020-01-01 | S-EPMC7640351 | BioStudies
2020-01-01 | S-EPMC7149931 | BioStudies
1000-01-01 | S-EPMC4598322 | BioStudies