Unknown

Dataset Information

0

In silico Evaluation of PLAC1-fliC As a Chimeric Vaccine against Breast Cancer


ABSTRACT: Background:Breast cancer is one of the most prevalent cancers among women. Common cancer treatment methods are not effective enough, and there is a need for a more efficient treatment procedure. Cancer vaccine is a novel immunotherapy method that stimulates humoral and/or cellular immunity against cancer. Placenta-specific protein 1 (PLAC1) is a cancer/testis antigen, prevalent in breast cancer and rarely found in normal tissues. FliC, as a bacterial adjuvant, when fused to PLAC1 can elicit humoral and cellular responses. Therefore, PLAC1-fliC is a chimeric protein, which can be considered a suitable candidate against breast cancer. Methods:ProtParam was used to evaluate the physicochemical properties of PLAC1-fliC. Second structures were determined using the GOR V server. PLAC1-fliC 3D structure was modeled by Phyre2, and it was refined using GalaxyWEB. The refined model was submitted to RAMPAGE, PROCHECK, and ProSA-web for validation. Antigenicity and allergenicity of the construct were predicted by ANTIGENpro, VaxiJen, AllergenFP, and SDAP databases. Then MHC-I- and MHC-II-binding epitopes of PLAC1-fliC were forecasted by NetMHC 4.0 and NetMHCII 2.3 Servers. Finally, Ellipro and CTLpred were employed to predict B-cell and cytotoxic T lymphocyte epitopes. Results:The construct was evaluated as a stable fusion protein, which could be antigenic and could stimulate B and T cells against breast cancer. Conclusion:PLAC1-fliC, as a cancer vaccine candidate, might be suitable and specific for breast cancer, which could evoke humoral and cellular immunity against this type of tumor.

SUBMITTER: Taheri-Anganeh M 

PROVIDER: S-EPMC7275624 | BioStudies | 2020-01-01

REPOSITORIES: biostudies

Similar Datasets

2016-02-24 | E-GEOD-78202 | BioStudies
2018-01-01 | S-EPMC6138762 | BioStudies
2016-02-24 | E-GEOD-78202 | ArrayExpress
2018-01-01 | S-EPMC6068355 | BioStudies
2013-01-01 | S-EPMC4235021 | BioStudies
2017-01-01 | S-EPMC5526067 | BioStudies
2009-01-01 | S-EPMC2781404 | BioStudies
2018-01-01 | S-EPMC5809008 | BioStudies
2020-01-01 | S-EPMC7244013 | BioStudies
2020-01-01 | S-EPMC6959440 | BioStudies