Unknown

Dataset Information

0

A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition.


ABSTRACT: There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, define correlates of immune protection, and down-select candidate antivirals. Here, we generate a highly infectious recombinant vesicular stomatitis virus (VSV) bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein and show that this recombinant virus, rVSV-SARS-CoV-2 S, closely resembles SARS-CoV-2 in its entry-related properties. The neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in a high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S, and neutralization of rVSV-SARS-CoV-2 S and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific therapeutics and for mechanistic studies of viral entry and its inhibition.

SUBMITTER: Dieterle ME 

PROVIDER: S-EPMC7332447 | BioStudies | 2020-01-01

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC7263493 | BioStudies
2020-01-01 | S-EPMC7594855 | BioStudies
2020-01-01 | S-EPMC7601831 | BioStudies
2020-01-01 | S-EPMC7390959 | BioStudies
2020-01-01 | S-EPMC7743076 | BioStudies
2020-01-01 | S-EPMC7409919 | BioStudies
2021-01-01 | S-EPMC7781002 | BioStudies
2020-01-01 | S-EPMC7239077 | BioStudies
2020-01-01 | S-EPMC7199903 | BioStudies
2020-01-01 | S-EPMC7461543 | BioStudies