Unknown

Dataset Information

0

Intracellular optical doppler phenotypes of chemosensitivity in human epithelial ovarian cancer.


ABSTRACT: Development of an assay to predict response to chemotherapy has remained an elusive goal in cancer research. We report a phenotypic chemosensitivity assay for epithelial ovarian cancer based on Doppler spectroscopy of infrared light scattered from intracellular motions in living three-dimensional tumor biopsy tissue measured in vitro. The study analyzed biospecimens from 20 human patients with epithelial ovarian cancer. Matched primary and metastatic tumor tissues were collected for 3 patients, and an additional 3 patients provided only metastatic tissues. Doppler fluctuation spectra were obtained using full-field optical coherence tomography through off-axis digital holography. Frequencies in the range from 10 mHz to 10 Hz are sensitive to changes in intracellular dynamics caused by platinum-based chemotherapy. Metastatic tumor tissues were found to display a biodynamic phenotype that was similar to primary tissue from patients who had poor clinical outcomes. The biodynamic phenotypic profile correctly classified 90% [88-91% c.i.] of the patients when the metastatic samples were characterized as having a chemoresistant phenotype. This work suggests that Doppler profiling of tissue response to chemotherapy has the potential to predict patient clinical outcomes based on primary, but not metastatic, tumor tissue.

SUBMITTER: Li Z 

PROVIDER: S-EPMC7562924 | BioStudies | 2020-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC4702146 | BioStudies
2020-01-01 | S-EPMC7506185 | BioStudies
2015-01-01 | S-EPMC4496219 | BioStudies
2019-01-01 | S-EPMC6463912 | BioStudies
2019-01-01 | S-EPMC6376473 | BioStudies
2019-01-01 | S-EPMC6548659 | BioStudies
2018-01-01 | S-EPMC6105510 | BioStudies
2018-12-31 | E-MTAB-6277 | ArrayExpress
2013-01-01 | S-EPMC3790792 | BioStudies
2020-01-01 | S-EPMC7318110 | BioStudies