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Modeling Parkinson's Disease Neuropathology and Symptoms by Intranigral Inoculation of Preformed Human ?-Synuclein Oligomers.

ABSTRACT: The accumulation of aggregated ?-synuclein (?Syn) is a hallmark of Parkinson's disease (PD). Current evidence indicates that small soluble ?Syn oligomers (?SynOs) are the most toxic species among the forms of ?Syn aggregates, and that size and topological structural properties are crucial factors for ?SynOs-mediated toxicity, involving the interaction with either neurons or glial cells. We previously characterized a human ?SynO (H-?SynO) with specific structural properties promoting toxicity against neuronal membranes. Here, we tested the neurotoxic potential of these H-?SynOs in vivo, in relation to the neuropathological and symptomatic features of PD. The H-?SynOs were unilaterally infused into the rat substantia nigra pars compacta (SNpc). Phosphorylated ?Syn (p129-?Syn), reactive microglia, and cytokine levels were measured at progressive time points. Additionally, a phagocytosis assay in vitro was performed after microglia pre-exposure to ?synOs. Dopaminergic loss, motor, and cognitive performances were assessed. H-?SynOs triggered p129-?Syn deposition in SNpc neurons and microglia and spread to the striatum. Early and persistent neuroinflammatory responses were induced in the SNpc. In vitro, H-?SynOs inhibited the phagocytic function of microglia. H-?synOs-infused rats displayed early mitochondrial loss and abnormalities in SNpc neurons, followed by a gradual nigrostriatal dopaminergic loss, associated with motor and cognitive impairment. The intracerebral inoculation of structurally characterized H-?SynOs provides a model of progressive PD neuropathology in rats, which will be helpful for testing neuroprotective therapies.


PROVIDER: S-EPMC7696693 | BioStudies | 2020-01-01

REPOSITORIES: biostudies

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